1 Modeling the Enzymatic Hydrolysis of Dilute - Acid Pretreated Douglas Fir
نویسنده
چکیده
Glucose yield from the enzymatic hydrolysis of cellulose was investigated as a function of cellulase enzyme loading (7-36 FPU/g cellulose) and solids concentration (7%-18% total solids) for up to 72 h on dilute sulfuric-acid pretreated Douglas Fir. The saccharification was performed on whole hydrolysate with no separation or washing of the solids. Enzyme loading had a significant effect on glucose yield; solids concentration had a much smaller effect even at higher glucose concentrations. The data were used to generate an empirical model for glucose yield, and to fit parameters of a cellulose hydrolysis kinetic model. Both models could be used for economic evaluation of a separate hydrolysis and fermentation process. Index Entries: cellulose hydrolysis, softwood, ethanol, enzyme kinetics, cellulase
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